Angana Chaudhuri

Angana Chaudhuri

A cheerfull girl with a lot of enthusiasm and hope

Publications

Fostering single cell oil synthesis by de novo and ex novo pathway in oleaginous microorganisms for biodiesel production

Published in Biofuels and Bioenergy:Opportunities and Challenges, 2021

This chapter explores biodiesel production using single cell oil (SCO) derived from oleaginous microorganisms such as yeasts, fungi, algae, and bacteria. The major drawback in SCO production is the high cost of the raw material, which further elevates the total production cost. Thus, the utilization of low-cost substrates, such as industrial wastes, can be a promising approach for an economical and sustainable biosynthesis of SCO. Oleaginous microorganisms can utilize both hydrophilic and hydrophobic substrates in two different biopathways: “de novo” and “ex novo” lipid fermentation, respectively. Besides SCO accumulation, these microorganisms have been extensively focused on their intrinsic capability of synthesizing a variety of high-value by-products such as lipases, biosurfactants, and organic acids. The SCO synthesized by lipid accumulating organisms can be transesterified to produce fatty acid methyl esters (FAMEs). The properties based on the FAMEs profile of the SCO predict its suitability for the production of biodiesel. Moreover, this chapter focuses on the opportunities and challenges of this SCO as a promising feedstock for biodiesel production.

Recommended citation: Panjanathan, R., Narayanan, S., Chaudhuri, A., Anjum, S., & Kandasamy, R. (2022). Fostering single cell oil synthesis by de novo and ex novo pathway in oleaginous microorganisms for biodiesel production. In Biofuels and Bioenergy (pp. 479–504). Elsevier. https://doi.org/10.1016/B978-0-323-85269-2.00018-6

A Sustainable Approach for the Synthesis of Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) Biocomposite by Employing Corncob-Derived Nanocellulose as a Reinforcing Agent

Published in Journal of Polymers and the Environment, 2021

Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) is a copolymer synthesized by Bacillus megaterium co-utilizing cheese whey and food waste hydrolysate for its one-step production. The optimized substrate ratio of 60:40 (v/v) manifested maximum biomass of 3.09 ± 0.12 g/L and PHBV yield of 2.0 ± 0.3 g/L. Batch kinetics study revealed maximum biomass and PHBV yield of 3.05 ± 0.07 g/L and 2.175 ± 0.06 g/L respectively, with 71.43 ± 0.28% g/g PHBV content. The integration of corncob-derived nanocellulose into PHBV was confirmed by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction (XRD) analysis. Thermogravimetric analysis (TGA) and differential scanning calorimetry (DSC) analyzed the thermal characteristics of the PHBV biocomposite, where the highest degradation temperature was obtained at 790 °C, thus exhibiting high thermal stability. The mechanical properties such as Young’s modulus, elongation at break, and tensile strength of the biocomposite was comparatively higher than PHBV and was found to be 40 MPa, 5.310%, and 11.110 MPa, respectively. The enhanced thermal and mechanical characteristics of PHBV biocomposite proves that the corncob-derived nanocellulose can be employed as a reinforcing agent.

Recommended citation: Narayanan, S., Anjum, S., Chaudhuri, A. et al. A Sustainable Approach for the Synthesis of Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) Biocomposite by Employing Corncob-Derived Nanocellulose as a Reinforcing Agent. J Polym Environ 29, 2080–2095 (2021). https://doi.org/10.1007/s10924-020-01994-3

Synthesis of single-cell oil by Yarrowia lipolytica MTCC 9520 utilizing slaughterhouse lipid waste for biodiesel production

Published in Biomass Conversion and Biorefinery, 2020

The present study explores biodiesel production from the single-cell oil (SCO) synthesized by Yarrowia lipolytica MTCC 9520, utilizing slaughterhouse lipid waste, goat tallow, as the carbon substrate. Various parameters, including cultivation time (96 h), pH(6), substrate concentration (1.5%, v/v), inoculum size (5%, v/v), and C/N ratio (100), were optimized to attain the maximum biomass and lipid yield and lipid content of 3.8 g/L, 2.6 g/L, and 69.3% (g/g dry weight), respectively. The presence of intracellular lipid bodies in Y. lipolytica was confirmed by observing the Nile red-stained cells by fluorescence microscopy. Besides, the fluorescence intensities of the intracellular lipid bodies were determined by the flow cytometer. The extracted SCO from Y. lipolytica MTCC 9520 was analyzed by Fourier transform infrared (FT-IR) spectroscopy. Gas chromatography-mass spectrometry (GC-MS) analysis of the transesterified SCO presented the fatty acid methyl ester (FAME) profile, including palmitic acid (42.9%), stearic acid (21.5%), myristic acid (18.3%), and oleic acid (7.0%). The obtained FAME composition was further used to predict the properties of the biodiesel. The revealed characteristics of the transesterified FAMEs signify the candidature of the synthesized SCO as an alternate feedstock for biodiesel production.

Recommended citation: Radha, P., Narayanan, S., Chaudhuri, A. et al. Synthesis of single-cell oil by Yarrowia lipolytica MTCC 9520 utilizing slaughterhouse lipid waste for biodiesel production. Biomass Conv. Bioref. (2020). https://doi.org/10.1007/s13399-020-01132-y

A Study on the Synthesis of Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) by Bacillus megaterium Utilizing Cheese Whey Permeate

Published in Journal of Polymers and the Environment, 2020

Cheese whey permeate, a processed by-product of the dairy industry, was utilized as the sole carbon source for the production of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) from Bacillus megaterium. In the present study, propionic acid was co-fed as an internal plasticizer to synthesize PHBV co-polymer. Cultivation parameters such as time, propionic acid, C/N ratio were optimized by investigating several parameters that affect the production of PHBV. The maximum PHBV content of 86.6% and the highest PHBV yield of 3.64 g/L were achieved at 72 h, 0.1% propionic acid content, and C/N ratio of 15. The presence of PHBV in the extracted sample was confirmed by gas chromatography–mass spectroscopy (GC–MS) and Fourier transform-infrared spectroscopy analysis, and further, the morphology was studied by scanning electron microscopy analysis. Mechanical characterization revealed that PHBV has a tensile strength of 4.41 MPa, which was higher than polyhydroxybutyrate with 3.37 MPa. The PHBV biopolymer was observed to be thermally stable between 100–400 °C. The melting point, crystallization temperature and glass transition temperature of PHBV were measured to be 116.60 °C, 108.92 °C and 87.88 °C respectively. The synthesized PHBV nanoparticles had an average diameter of 343.3 nm, and the zeta potential was found to be—21.3 mV.

Recommended citation: Suhazsini, P., Keshav, R., Narayanan, S. et al. A Study on the Synthesis of Poly (3-hydroxybutyrate-co-3-hydroxyvalerate) by Bacillus megaterium Utilizing Cheese Whey Permeate. J Polym Environ 28, 1390–1405 (2020). https://doi.org/10.1007/s10924-020-01687-x